Title of Paper:Analysis of Cell Dielectrophoretic Motion with Microfluidic Device Embedding Multi-electrodes

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Affiliation of Author(s):机电学院

Journal:CHINESE JOURNAL OF ANALYTICAL CHEMISTRY

Key Words:Dielectrophoresis Cell motion Multi-electrode Composite electric field Optimal electrode combination Microfluidic chip

Abstract:The motion characteristics of different cells in the multi-electrode array microchip were studied under the dielectrophoretic force , and the two cells with the same external morphology but different internal histone protein were separated. The multi-electrode array microfluidic chip was embedded in the 5 square cross section of the channel , and 8 electrodes were embedded on each side of the cross section. The structure enlarged the size of the microfluidic channel , which was beneficial for the high flow separation of the cells under the action of the dielectrophoretic force. To study the characteristics of cell motion in the microfluidic device , the electric field distribution in a cross-section was calculated , and the optimal electrode combination pattern was obtained , which generated a uniform electric field distribution and a greatest the dielectrophoretic force. After that, the motion characteristics of MRC-5 in human lung fibroblasts in different frequencies and complex electric fields at different frequencies and complex electric fields were analyzed. By analyzing the spectrum of the dielectrophoretic force , the separation frequency of the two kinds of cells of wild type (WT) and histone-GFP type was f = 30 kHz, and the separation rate of cell was calculated by adding different proportions of sucrose solution and two cell mixture at two inlets. That was, when the flow-rate ratio of the 2 inlets was 12:1 , the separation rate of the two types of cells was 93. 5% . The method for cell separation using microfluidic chips provided a basis for the rapid separation of cells in the future.

ISSN No.:0253-3820

Translation or Not:no

Date of Publication:2019-02-01

Co-author:Jiang Zhu-Peng,Zhao Tong,Wang Hao,Chen Bai,wht

Correspondence Author:Jiafeng YAO