Affiliation of Author(s):自动化学院
Journal:华中科技大学学报(医学版)
Key Words:神经干细胞;贴壁培养;包被处理;免疫荧光;悬浮培养;
Abstract:目的探索一种改进的贴壁培养神经干细胞的方法。方法从胚胎期为15.5d的胎鼠大脑皮层中提取神经干细胞,在PO/FN(Poly-L-ornithine hydrochloride/Fibronectin)包被处理后的培养瓶中进行贴壁培养并传代,在倒置显微镜下观察神经干细胞的细胞形态,并通过免疫荧光法鉴定神经干细胞及其子代细胞进行分化操作之后的情况。结果原代神经干细胞细胞形态清晰,免疫荧光标记物明显。结论相对于传统神经球悬浮培养法该法处理简便,传代损耗低,容易观察到细胞形态和分化阶段,更有助于神经干细胞的培养和存储。
Translation or Not:no
Date of Publication:2017-06-15
Co-author:杨骐昌,韩平
Correspondence Author:Song Xiao Feng
Professor
Supervisor of Doctorate Candidates
Gender:Male
Alma Mater:浙江大学
Education Level:浙江大学
Degree:Doctoral Degree in Engineering
School/Department:College of Automation Engineering
Discipline:Measuring and Testing Technologies and Instruments. Precision Instrument and Machinery
Business Address:自动化学院2号楼408
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